mv3 melanoma cells  (Eppendorf AG)

Journal: Scientific Reports

Article Title: α-terpineol induces apoptosis in melanoma cells and its underlying mechanism

doi: 10.1038/s41598-025-34538-7

Figure Lengend Snippet: α-Terpineol inhibits the proliferation and clonogenicity of A375 and MV3 melanoma cells. (A) Chemical structure of α-terpineol; (B , C) A375 and MV3 cell viability post 48-hour α-terpineol treatment; (D) Inhibitory effects of 6.16 mg·L⁻¹ α-terpineol, 12.32 mg·L⁻¹ α-terpineol, and Vemurafenib on the proliferation of A375 and MV3 cells (Magnification: 100x). (E) Inhibitory effects of 6.16 mg·L⁻¹ α-terpineol, 12.32 mg·L⁻¹ α-terpineol, and Vemurafenib on colony formation of A375 and MV3 cells. ( **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, and ns p ≥ 0.05). The concentration of DMSO was 20 µg·L⁻¹. All experiments were repeated three times.

Article Snippet: Human melanoma A375 and MV3 cell lines were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and cultured in high-glucose DMEM medium (Invitrogen Gibco, Carlsbad, CA, USA) with 10% fetal bovine serum (Wuhan Pricella Biotechnology Co., Ltd.) and 1% penicillin-streptomycin (Solarbio Life Science, Beijing, China).

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: α-terpineol induces apoptosis in melanoma cells and its underlying mechanism

doi: 10.1038/s41598-025-34538-7

Figure Lengend Snippet: α-Terpineol inhibits migration and invasion in A375 and MV3 melanoma cells. (A) Effects of α-terpineol (6.16 and 12.32 mg·L⁻¹) and Vemurafenib on the migration of A375 and MV3 cells (Magnification: 100x); (B) Inhibitory effects of α-terpineol (6.16 and 12.32 mg·L⁻¹) and Vemurafenib on the invasion of A375 and MV3 cells (Magnification: 200x). ( **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns p ≥ 0.05). The concentration of DMSO was 20 µg·L⁻¹. All experiments were repeated three times.

Article Snippet: Human melanoma A375 and MV3 cell lines were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and cultured in high-glucose DMEM medium (Invitrogen Gibco, Carlsbad, CA, USA) with 10% fetal bovine serum (Wuhan Pricella Biotechnology Co., Ltd.) and 1% penicillin-streptomycin (Solarbio Life Science, Beijing, China).

Techniques: Migration, Concentration Assay

Journal: Scientific Reports

Article Title: α-terpineol induces apoptosis in melanoma cells and its underlying mechanism

doi: 10.1038/s41598-025-34538-7

Figure Lengend Snippet: α-Terpineol induces apoptosis in A375 and MV3 melanoma cells. (A) Detection of apoptosis by AO/EB staining in A375 and MV3 cells after 48-hour treatment with α-terpineol (6.16 and 12.32 mg·L⁻¹) or Vemurafenib (Magnification: 100x); (B) Analysis of cell cycle distribution in A375 and MV3 melanoma cells treated with α-terpineol (6.16 and 12.32 mg·L⁻¹) or Vemurafenib for 48 h; (C) Flow cytometry was used to quantitatively analyze the apoptosis rate of A375 and MV3 cells after a 48-hour treatment with α-terpineol (6.16 and 12.32 mg·L⁻¹) or Vemurafenib; (D) Western blot analysis showing expression changes of apoptosis-related proteins (Bax, Bcl-2, caspase-3, cleaved caspase-3, caspase-8, and cleaved caspase-8) in A375 and MV3 cells after 48-hour treatment with α-terpineol (6.16 mg·L⁻¹ and 12.32 mg·L⁻¹) ( **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns p ≥ 0.05). The concentration of DMSO was 20 µg·L⁻¹. All experiments were repeated three times.

Article Snippet: Human melanoma A375 and MV3 cell lines were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and cultured in high-glucose DMEM medium (Invitrogen Gibco, Carlsbad, CA, USA) with 10% fetal bovine serum (Wuhan Pricella Biotechnology Co., Ltd.) and 1% penicillin-streptomycin (Solarbio Life Science, Beijing, China).

Techniques: Staining, Flow Cytometry, Western Blot, Expressing, Concentration Assay

Journal: Scientific Reports

Article Title: α-terpineol induces apoptosis in melanoma cells and its underlying mechanism

doi: 10.1038/s41598-025-34538-7

Figure Lengend Snippet: Predicted binding interactions of α-terpineol with key target proteins and its facilitation of apoptosis in A375 and MV3 melanoma cells. (A) Molecular docking images of α-terpineol with JAK2 and STAT3; (B) Impact of α-terpineol (6.16 mg·L⁻¹ and 12.32 mg·L⁻¹) over 48 h on JAK2, p-JAK2, STAT3, and p-STAT3 protein levels in A375 and MV3 cells ( **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, and ns p ≥ 0.05).

Article Snippet: Human melanoma A375 and MV3 cell lines were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and cultured in high-glucose DMEM medium (Invitrogen Gibco, Carlsbad, CA, USA) with 10% fetal bovine serum (Wuhan Pricella Biotechnology Co., Ltd.) and 1% penicillin-streptomycin (Solarbio Life Science, Beijing, China).

Techniques: Binding Assay